The role of IL-1β in the development of diabetes mellitus

Background. Currently, diabetes mellitus is the most common of all endocrine diseases with increasing tendency. The purpose our research was to study dynamics serum levels proinflammatory cytokine-IL-1β in streptozotocin-induced diabetes. Materials and methods. experiments were performed on 88 white male Wistar rats weighing 170-210 g. Animals divided into three groups: 1 - intact; 2 control; 3 experimental a model mellitus, which reproduced by intraperitoneal injection streptozotocin “Sigma" company (USA), diluted 0.1 M citrate buffer pH 4.5, at rate 60 mg/kg body weight. control group animals received an equivalent dose solution 4.5.
 All studies under thiopental-sodium anesthesia Serum IL-1β determined enzyme-linked immunosorbent assay (ELISA) kit (Elabscience, USA) according manufacturer's instructions 14, 28, 42 70 days after injection. STATISTICA 10 program used for statistical processing obtained results. Results. Conducted biochemical analysis showed that there increase content cytokine compared similar indicators stages experiment: 14 22.5%, 28 40.2%, 72.8% 107.2%. Conclusion. results suggest pro-inflammatory interleukin-1β plays one leading roles pathogenesis diabetes, as indicated significant this experiment.